UCT EMU held the first CCP4 Crystallographic School in South Africa – Data Collection to Structure Refinement and Beyond, from 22 February – 5 March 2021 at the University of Cape Town, South Africa. This workshop was organized jointly by Trevor Sewell (University of Cape Town, South Africa), Carmien Tolmie (University of the Free State, South Africa), Gwyndaf Evans (Diamond Light Source, UK), Ronan Keegan (CCP4, STFC Rutherford Appleton Laboratory, UK), Marco Mazzorana (Diamond Light Source, UK), Elliot Nelson (Diamond Light Source, UK), Felicity Bertram (Diamond Light Source, UK) with the help of Ruslan Sanishvili (USA).
The workshop covered all aspects of protein structure solution by X-ray crystallography, including the fundamental concepts of protein crystallography, crystallographic data collection, processing of diffraction data, phasing, structure refinement, validation and deposition in the PDB, as well as an introduction to CryoEM and Electron Diffraction.
Participants had the opportunity to collect data from their own crystals at Diamond Light Source via remote access, in a data collection session facilitated by Diamond beamline staff. Software covered includes iMosflm, XDS, DIALS, BLEND, PHASER, SHELX, MrBUMP, SIMBAD, ARP/wARP, Coot, REFMAC, Isolde, Crank2, PISA and more. This workshop is most suitable for postgraduate students, postdoctoral researchers and early career scientists working in structural biology.
There is no registration fee for the workshop however, the number of applicants selected for participation will be limited to 25.